RESUMO
An HPLC method for the separation and the quantitative determination of flavonol glycosides, acylflavonol glycosides, and biflavones in crude leaf extracts from GINKGO BILOBA is described. The results, expressed in percentage of rutine, kaempferol P-coumaroyl glucorhamnoside, and bilobetin showed a higher amount of acylflavonol glycosides in buds, of flavonol glycosides in spring leaves, and of biflavones in autumn leaves.
RESUMO
Two new saponins were isolated from Mimosa tenuiflora and their structures established as 3-O-[alpha-L-rhamnopyranosyl(1----2)-beta-D-glucopyranosyl-(1----3]-(alp ha-L- arabinopyranosyl-(1----4]-beta-D-xylopyranosyl-(1----2)]-[beta-D- xylopyranosyl-(1----4)]-beta-D-glucopyranosyl)-28-O-alpha-L-rhamnopyrano syl oleanolic acid and 3-O-[alpha-L-rhamnopyranosyl-(1----2)-beta-D-glucopyranosyl-(1----3]-(al pha- L-arabinopyranosyl-(1----4]beta-D-xylopyranosyl-(1----2)]-[beta-D- xylopyranosyl-(1----4)]-beta-D-glucopyranosyl) oleanolic acid.
Assuntos
Ácido Oleanólico/análogos & derivados , Plantas Medicinais/química , Saponinas/isolamento & purificação , Sequência de Carboidratos , Hidrólise , Espectroscopia de Ressonância Magnética , Metilação , Dados de Sequência Molecular , Saponinas/químicaRESUMO
The antagonistic activity of various thiol compounds versus the cytotoxic effects of valtrate and didrovaltrate has been evaluated on cultured hepatoma cells. Compounds with free SH groups like cysteine, mercaptoethanol, dithioerythritol, and glutathione were able to suppress the cytotoxicity of the valepotriates in a dose-dependent way, whereas compounds with blocked SH groups did not antagonize these toxic effects. The possible interactions between the valepotriates and thiol compounds are discussed.
Assuntos
Citotoxinas/antagonistas & inibidores , Iridoides , Fígado/efeitos dos fármacos , Extratos Vegetais/antagonistas & inibidores , Piranos/antagonistas & inibidores , Compostos de Sulfidrila/farmacologia , Animais , Extratos Vegetais/toxicidade , Piranos/toxicidade , Ratos , Células Tumorais CultivadasRESUMO
Ginkgo biloba extract is known to be efficient in diseases associated with free radical generation. The purpose of this work was to study, under in vitro conditions, the action of Ginkgo biloba extract (Gbe) against superoxide anion (O2-.), which is directly or indirectly implicated in cell damage. Gbe appears to have both an O2-. scavenging effect and also a superoxide dismutase activity. Its antiradical effect was demonstrated by low temperature electron spin resonance and in a non-enzymatic system (phenazine methosulfate-NADH), and its enzymatic activity was shown by polarographic determination.
Assuntos
Extratos Vegetais/farmacologia , Superóxido Dismutase/antagonistas & inibidores , Superóxidos/antagonistas & inibidores , Animais , Ânions , Bovinos , Eletroquímica , Espectroscopia de Ressonância de Spin Eletrônica , Radicais Livres , Superóxido Dismutase/metabolismoRESUMO
6-Hydroxykynurenic acid and nine flavonol glycosides were isolated and identified from the leaves of GINKGO BILOBA: three of them were identified in Ginkgo for the first time, and two others are unusual P-coumaroyl esters.
RESUMO
Haitian empirical medicine sprang from both European (16th to 19th century) and African (especially voodoo) traditional therapies. The use of medicinal herbs is highly developed. Our purpose was to list the plants held to be antifertility agents in the island. We identified about twenty species more or less currently used by the women as abortifacients or emmenagogues. The chemistry and active components of a few species are well-known. However, for most of them, some were partially studied, and no relation could be established between their chemical composition and their potential activities, and the rest are chemically unknown. We chemically screened extracts of Casearia ilicifolia, Eleutherine bulbosa, Rhoeo spathacea and Stemodia durantifolia, and identified flavonoids, triterpenes and sterols in the leaves of C. ilicifolia, and naphthoquinones, and a new anthraquinone, anthracene-9,10-dione-1,5-diol-4-methoxy-3-methyl-2-carboxylic acid methyl ester, in the bulbs of E, bulbosa. R. spathacea showed a stimulative activity on mouse uterus. Antifertility screening tests of C. ilicifolia and E. bulbosa showed activity in rats, but also probably toxicity.
Assuntos
Anticoncepcionais , Plantas Medicinais , Animais , Fenômenos Químicos , Química , Anticoncepcionais/isolamento & purificação , Cultura , Implantação do Embrião/efeitos dos fármacos , Feminino , Haiti , Hormônios/metabolismo , Humanos , Magia , Camundongos , Ocitócicos , Plantas Medicinais/análise , Gravidez , RatosRESUMO
Haitian empirical medicine sprang from both European (16th to 19th century) and African (especially voodoo) traditional therapies. The use of medicinal herbs is highly developed. Our purpose was to list the plants held to be antifertility agents n the island. We identified about twenty species more or less currently used by the women as abortifacients or emmenagogues. The chemistry and active components of a few species are well-known. However, for most of them, some were partially studied, and no relation could be established between their chemical composition and their potential activities, and the rest are chemically unknown. We chemically screened extracts of casearia ilicifolia, Eleutherine bulbosa, Rhoeo spathacea and the leaves of C.ilieifolia, and naphthoquinones, and a new anthraquinine, anthracene -9, 10-dione-1, 5-diol-4-methoxy-3-methyl-2-carboxylec acid methyl ester, in the bulbs of E. bulbosa, R.spathacea showed a stimulative activity on mouse uterus. Antifertility screening tests of C.ilieifolia and E. bulbosa showed activity in rats, but also probably toxicity
